Data provided by the SPEX Fluorescence Group, Horiba Jobin Yvon Inc. Ann Neurol 71, 805–814 (2012).60.Staley, K. J. & Dudek, F. E. Interictal spikes and epileptogenesis. Entorhinal cortical defects in Tg2576 mice are present as early as 2–4 months of age. Raw FFT power spectra were then standardized by zscore in the frequency domain using the mean and standard deviation of the EEG during periods of quiet wakefulness within a given recording session.
Blue: Mean IIS frequency (line) and variance (shading) increases with age and becomes variable. The fluorescence quantum yield of Alexa Fluor® 568 carboxylic acid, succinimidyl ester in 50 mM potassium phosphate, 150 mM NaCl pH 7.2 at 22°C is 0.69. The fluorescence lifetime (τ) of the Alexa Fluor® 594 dye in pH 7.4 buffer at 20°C is 3.9 nanoseconds. For data sets with zero values or a failed Lillefors test of normality (p < 0.05), non-parametric tests were used (Mann-Whitney for 2 groups and Kruskal-Wallis for > 2 groups with Dunn-Sidak post hoc tests).How to cite this article: Kam, K. et al. Fluorescence intensities were measured for equal concentrations of the three dyes using excitation/emission at 490/520 nm. Neuroscience 164, 1334–1346 (2009).36.Arnaud, C., Gauthier, P. & Gottesmann, C. Atropine effects on the intermediate stage and paradoxical sleep in rats.
Figure 1.3.11 Brightness comparison of Alexa Fluor® 555 goat anti–mouse IgG antibody with commercially available Cy3 goat anti–mouse IgG antibody conjugates. Both Alexa Fluor® 488 carboxylic acid TFP ester and Alexa Fluor® 488 SDP ester are stable for several hours at the basic pH typically used for reactions—far outlasting succinimidyl esters. Epileptiform discharges and seizures were absent at 5–7 weeks of age, but did occur at older ages. Neurobiol Aging 36, 134–148 (2015).17.Iyengar, S. S. et al. For each behavioral state (from left to right, NREM, REM, quiet wakefulness, active wakefulness) the FC recording of the EEG is shown at the top, with the spectrogram corresponding to it shown below. To the right is the power spectrum. Red blood cells were lysed, and the samples were analyzed with a flow cytometer equipped with a 488 nm argon-ion laser and a 525 ± 10 nm bandpass emission filter.